Determination of quantum dots and their conjugates by size exclusion chromatography
Keywords:
quantum dots, conjugates of quantum dots with monoclonal antibodies, exclusive chromatography, mobile phaseAbstract
Quantum dot conjugates (QDs) with proteins can be used in a wide variety
of bioanalysis applications – from immunochemical test methods to visualization of affected tissues and tracking of
drugs in the body. The increasing expansion of the potential for the practical application of CT
and their conjugates with biomolecules makes it necessary to search for reliable and effective methods of analysis that allow identifying analytes, determining their quality and tracking
degradation processes during storage and during operation. The use of exclusive chromatography allows us to evaluate the size, uniformity, aggregation processes of synthesized CT, monoclonal antibodies and their conjugates. In this paper, the choice of conditions for chromatographic
analysis, under which high selectivity and efficiency of separation of the objects of study is observed, is carried out. It is demonstrated that the use of an anionic surfactant additive
in the mobile phase, it allows to significantly reduce the interaction of analytes with the stationary phase and makes it possible to evaluate their sizes
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