Research of the process of isolation of gliadin from the tritikale grain culture and development of a sample identification method using capillary electrophoresis
Keywords:
triticale, triticale flour, protein identification, capillary electrophoresis, gliadinAbstract
The present article deals with the triticale grain flour obtained by direct grinding. For the extraction of plant protein from flourgliadin, the modernized Osborne method was applied, which consists in the stepwise washing of fractions with water, alcohol, benzene and ethyl ether. The obtained protein was studied by capillary electrophoresis. The electropherogram contains a number of compounds with similar molecular weight
References
Бутковский В.А., Мельников Е.М. Технология мукомольного, крупяного и комбикормового производства. М.: Агропромиздат, 1989. 464 с.
Витол И.С., Карпиленко Г.П., Кандроков Р.Х., Стариченков А.А., Коваль А.И., Жильцова Н.С. Белково-протеиназный комплекс зерна тритикале // Хранение и переработка сельхозсырья. 2015. № 8. С. 36–39.
Леонова С.А., Погонец Е.В. Технология крупы из пророщенного зерна тритикале // Технология и товароведение
инновационных пищевых продуктов. 2016. № 1(36). С. 30–33.
Погонец Е.В., Леонова С.А. Характеристика технологических свойств тритикале сорта Башкирская короткостебельная // Зерновое хозяйство России. 2011. № 3. С. 63–67.
Чумикина Л.В., Арабова Л.И., Колпакова В.В., Топунов А.Ф. Активность ферментов обмена глутамина в прорастающем зерне тритикале // Физиология растений и генетика. 2013. Т. 45, № 5. С. 390–398.
Aprodu I., Banu I. Comparative analyses of physicochemical and technological properties of triticale, rye and wheat // Annals of the University Dunarea De Jos of Galati, Fascicle Vi-Food Technology. 2016. T. 40, № 2. C. 31–39.
Bellil I., Bouguennec A., Khelifi D. Diversity of Seven Glutenin and Secalin Loci within Triticale Cultivars Grown in France // Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2010. T. 38, № 2. C. 48–55.
Jiang Q.T., Wei Y.M., Andre L., Lu Z.X., Pu Z.E., Peng Y.Y., Zheng Y.L. Characterization of omega-secalin genes from rye, triticale, and a wheat 1BL/1RS translocation line // Journal of Applied Genetics. 2010. T. 51, № 4. C. 403–411.
Lopez A.M.M., Ohm J.-B., Manthey F.A., Rao J., Simsek S. Gluten extraction from deoxynivalenol contaminated wheat by wet milling // Food Control. 2021. T. 120. C. 2.
Mehanna M.M., Mneimneh A.T. Updated but not outdated «Gliadin»: A plant protein in advanced pharmaceutical nanotechnologies // International Journal of Pharmaceutics. 2020. T. 587. C. 34.
Munoz-Insa A., Gastl M., Becker T. Influence of Malting on the Protein Composition of Triticale (x Triticosecale Wittmack) 'Trigold' // Cereal Chemistry. 2016. T. 93, № 1. C. 10–19.
Prabucka B., Drzymaia A., Grabowska A. Molecular cloning and expression analysis of the main gliadin-degrading cysteine endopeptidase EP8 from triticale // Journal of Cereal Science. 2013. T. 58, № 2. C. 284–289.
Salmanowicz B.P., Nowak J. Diversity of Monomeric Prolamins in Triticale Cultivars Determined by Capillary Zone Electrophoresis // Journal of Agricultural and Food Chemistry. 2009. T. 57, № 6. C. 2119–2125.
